Our team Biomolecules: Analysis, Molecular and Cellular Interactions is multidisciplinary with expertise in biology, chemistry and physics. We are interested in developing biophysical and chemical biology tools to understand the functions of biomolecules and their interactions in various biological processes. Our projects focus on:

  • The fundamental understanding of functional biomolecules, such as cell-penetrating peptides (CPPs), antimicrobial peptides (AMPs), homeoproteins (HPs) and viral peptides, and their general utilization for biotechnological and biomedical applications (F. Burlina, S. Cribier, T. Drujon, F. Illien, C. Lacombe, D. Ravault, N. Rodriguez, E. Sachon, S. Sagan, A. Walrant, D. Warschawski);
  • The design of photolabelling and bioorthogonal ligation-based approaches to study post-translational and epigenetic modifications (G. Bolbach, F. Burlina, T. Drujon, E. Sachon), and the use of extended chemical ligation for the synthesis of modified proteins (F. Burlina);

  • The development of new analytical tools such as mass spectrometry-based methods for the detection of biomolecule interactions in complex systems (G. Bolbach, E. Sachon, S. Sagan, A. Walrant), solid-state NMR-based methods to study living micro-organisms such as bacteria or micro-algae and the effects of pollution (D. Warschawski), and original biophysical setups to measure biomolecular interactions and movements of charged molecules through membranes (S. Cribier, N. Rodriguez);

  • The invention of new fluorescent biosensors and chemical-genetic tools to observe and study dynamic biochemical events in live cells and tissues with high spatial and temporal resolution (A. Gautier, N. Pietrancosta).

GDR 3625 MuFoPam 

GDR 3739 GAGoSciences 

GDR 3070 CellTiss

GDR Approches quantitatives du vivant

GDR Chemobiologie (soon to be created)

Peptide synthesis, HPLC

Cell culture: bacteria, eukaryote

Protein expression and purification by FPLC

Protein engineering: electrophoresis, electrotransfer, immunodetection, in-gel digestion for MS analysis

Molecular biology: cloning

Purification biotin/streptavidin, C18, C8, TiO2, Ni2+, Co2+

Microscopy: evanescent wave, phase contrast, fluorescence

Flow cytometry

Photoaffinity labeling

Fluorescence, absorbance spectroscopy

Fluorescence Recovery After Photobleaching (FRAP)

Calorimetry: Isothermal Titration; Differential Scanning

Model membrane: GUV, MLV, LUV

Mass spectrometry: MALDI-TOF, TOF-TOF, nanoLC-ESI

Antimicrobial assays

ANR DKP-COMBIBIO 2017-2020 (Coordinator: P. Belin; Partner: E. Sachon)

ANR TP-Peptides 2017-2020 (Coordinator: P. Dalko; Partner: F. Burlina)

ANR CROSS 2018-2021 (Coordinator: A. Joliot; Partner: S. Sagan)

ANR ADOBE 2020-2023 (Coordinator: Nicolas Despras; Partner: A. Gautier)

ANR AUDITIF 2020-2023 (Coordinator: Frédéric Olivier; Partner: D. Warschawski)

ERC Fluoswitch 05/2017-05/2022 (Coordinator: A. Gautier)

Fondation ARC 01/2019-12/2020 (Coordinator: S. Sagan)

PHC Balaton 01/2019-12/2020 (Coordinators: Z. Banoczi (Hungary) S. Sagan (France))

SATT Lutech Peptides antimicrobiens et leurs utilisations (Coordinator: C. Lacombe, E. Sachon)

80 Prime CNRS (Coordinator: Nicolas Pietrancosta)

Les membres du pôle

Les étudiants du pôle